PLS-DA (Partial Least Squares Discriminant Analysis)

Discriminate groups in omics or high-dimensional data.

Definition

PLS-DA is a supervised method combining dimensionality reduction (like PCA) with group discrimination. It is particularly used in metabolomics, proteomics, and genomics where the number of variables far exceeds the number of observations.

When to use it

Requirements

What StatsLab computes

Worked example

Context : Discriminating 3 cancer types (n=90) from 150 blood metabolites.

Result : LV1: 28% variance · LV2: 19% · Clear separation of 3 groups on scores plot

Interpretation : PLS-DA perfectly discriminates the 3 cancer types on the first 2 components. The 15 metabolites with VIP > 1.5 are the most relevant candidate biomarkers.

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